b16f10 atcc 6475 experimental models Search Results


mouse melanoma cell b16  (ATCC)
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ATCC mouse melanoma cell b16
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crl 6475  (ATCC)
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ATCC crl 6475
Crl 6475, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b16f10luc2 cells  (ATCC)
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ATCC b16f10luc2 cells
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b16 f10  (ATCC)
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ATCC b16 f10
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b16f10 crl 6475 cell lines  (ATCC)
99
ATCC b16f10 crl 6475 cell lines
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
B16f10 Crl 6475 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse melanoma line  (ATCC)
97
ATCC mouse melanoma line
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
Mouse Melanoma Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse melanoma line - by Bioz Stars, 2026-08
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eukaryotic cell lines  (ATCC)
99
ATCC eukaryotic cell lines
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
Eukaryotic Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b16f10+atcc+6475+experimental+models/pmc05581859-237-3-7?v=ATCC
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eukaryotic cell lines - by Bioz Stars, 2026-08
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crl 6475 crl 2639  (ATCC)
95
ATCC crl 6475 crl 2639
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
Crl 6475 Crl 2639, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b16f10 cells # crl-6475  (LGC Promochem)
90
LGC Promochem b16f10 cells # crl-6475
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
B16f10 Cells # Crl 6475, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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6475 mcf7 human breast carcinoma atcc cat  (ATCC)
99
ATCC 6475 mcf7 human breast carcinoma atcc cat
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
6475 Mcf7 Human Breast Carcinoma Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines b16 f0 atcc crl 6322 b16 f10 atcc crl 6475 ln lines  (ATCC)
96
ATCC cell lines b16 f0 atcc crl 6322 b16 f10 atcc crl 6475 ln lines
Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with <t>B16F10</t> in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.
Cell Lines B16 F0 Atcc Crl 6322 B16 F10 Atcc Crl 6475 Ln Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with B16F10 in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.

Journal: Frontiers in Immunology

Article Title: Potentiating the Antitumor Activity of Cytotoxic T Cells via the Transmembrane Domain of IGSF4 That Increases TCR Avidity

doi: 10.3389/fimmu.2020.591054

Figure Lengend Snippet: Overexpression of IG4ΔEXT enhances the antitumor activity of CD8 + T cells in vitro and in vivo . (A) Efficiency of the retroviral transduction of EV or IG4ΔEXT in CD8 + T cells from OTI mouse. (B) EV- or IG4ΔEXT- OTI CD8 + T cells were incubated with B16F10 in the presence or absence of OVA 257-265 for 9 h, and then the surface expression of CD69 was measured. IFN-γ and TNF-α were measured at 24 h post-incubation with B16F10 in the presence or absence of OVA peptide. * P < 0.001 vs. EV- OTI CD8 + T cells. (C) Target cells from (B) were stained with 7-AAD, and dead cells were counted by flow cytometry. (B, C) Data represent the means of three independent experiments. * P < 0.001 vs. EV- OTI CD8 + T cells (D) Schematic diagram of the metastasis and solid tumor mouse models (left). Gross and H&E staining images of B16F10 lung metastatic nodules from C57BL/6 mice administered via IV injection with PBS, EV-, or IG4ΔEXT- OTI CD8 + T cells (right). Arrowheads indicate metastatic foci. The metastatic colonies were quantified and represented as a bar graph. Data represent the means of three independent experiments (n=4 mice per group). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells. (E) Images of B16F10 solid tumors obtained from mice. Tumor volumes were measured using calipers every 3 days after PBS or T cell (EV -OTI CD8 + T, wt-IG4- OTI CD8 + T, and IG4ΔEXT- OTI CD8 + T) injection until day 21. (F) In some cases, tumors were harvested at day 14 from the mice described above (E) . Tumor weights were measured at the end of the experiments. Data are representative of four independent experiments (n=6 mice per group for each experiment). * P < 0.005 vs. PBS, ** P < 0.001 vs. EV- OTI CD8 + T cells.

Article Snippet: Jurkat T (TIB-152), HEK293T (CRL-1573), and B16F10 (CRL-6475) cell lines were purchased from ATCC.

Techniques: Over Expression, Activity Assay, In Vitro, In Vivo, Retroviral, Transduction, Incubation, Expressing, Staining, Flow Cytometry, IV Injection, Injection